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Vet Immunol Immunopathol. 2014 Dec 15;162(3-4):198-202. doi: 10.1016/j.vetimm.2014.10.008. Epub 2014 Oct 26.

The evaluation of candidate biomarkers of cell-mediated immunity for the diagnosis of Mycobacterium bovis infection in African buffaloes (Syncerus caffer).

Author information

1
DST/NRF Centre of Excellence for Biomedical TB Research/MRC Centre for Tuberculosis Research/Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 19063, Tygerberg 7505, South Africa.
2
Ezemvelo KZN Wildlife, PO Box 25, Mtubatuba 3935, South Africa.
3
DST/NRF Centre of Excellence for Biomedical TB Research/MRC Centre for Tuberculosis Research/Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 19063, Tygerberg 7505, South Africa. Electronic address: sparsons@sun.ac.za.

Abstract

We evaluated commercially available bovine enzyme linked immunosorbent assays (ELISA) and a human IP-10 ELISA to measure IP-10, MIG, MCP-1, MCP-2, MCP-3 and IL1-RA in buffalo plasma in order to identify sensitive markers of the immune response to Mycobacterium bovis-specific peptides. Additionally, we found that all coding mRNA sequences of these cytokines showed very high homology with their homologues in domestic cattle (97-99%) as did the derived amino acid sequences (97-99%). This high sequence homology between cattle and buffaloes supports the use of bovine ELISAs for the detection these cytokines in buffaloes. MCP-1 concentration showed a positive correlation with that of IFN-γ (p=0.0077) and appears to occur in far greater abundance in buffaloes when compared to humans. Using a bovine IP-10 ELISA, levels of this cytokine were found to be significantly increased in antigen-stimulated blood samples from M. bovis test positive buffaloes (p<0.0001) and IP-10 was detected in far greater abundance than IFN-γ. Measurement of IP-10 with this ELISA may prove to be a sensitive marker of M. bovis infection in African buffaloes.

KEYWORDS:

African buffalo; Bovine tuberculosis; Enzyme linked immunosorbent assay; Interferon gamma; Interferon gamma-induced protein 10

PMID:
25464825
DOI:
10.1016/j.vetimm.2014.10.008
[Indexed for MEDLINE]

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