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Neuroimage. 2015 Jan 15;105:486-92. doi: 10.1016/j.neuroimage.2014.11.010. Epub 2014 Nov 8.

Validation of quantitative susceptibility mapping with Perls' iron staining for subcortical gray matter.

Author information

1
Department of Biomedical Engineering, University of Alberta, Edmonton, Alberta, Canada, T6G 2V2.
2
Division of Neurology, Department of Medicine, University of Alberta, Edmonton, Alberta, Canada, T6G 2G3.
3
Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada, T6G 2B7.
4
Department of Radiology and Diagnostic Imaging, University of Alberta, Edmonton, Alberta, Canada, T6G 2B7.
5
Department of Biomedical Engineering, University of Alberta, Edmonton, Alberta, Canada, T6G 2V2. Electronic address: wilman@ualberta.ca.

Abstract

Quantitative susceptibility mapping (QSM) measures bulk susceptibilities in the brain, which can arise from many sources. In iron-rich subcortical gray matter (GM), non-heme iron is a dominant susceptibility source. We evaluated the use of QSM for iron mapping in subcortical GM by direct comparison to tissue iron staining. We performed in situ or in vivo QSM at 4.7 T combined with Perls' ferric iron staining on the corresponding extracted subcortical GM regions. This histochemical process enabled examination of ferric iron in complete slices that could be related to susceptibility measurements. Correlation analyses were performed on an individual-by-individual basis and high linear correlations between susceptibility and Perls' iron stain were found for the three multiple sclerosis (MS) subjects studied (R(2) = 0.75, 0.62, 0.86). In addition, high linear correlations between susceptibility and transverse relaxation rate (R2*) were found (R(2) = 0.88, 0.88, 0.87) which matched in vivo healthy subjects (R(2) = 0.87). This work validates the accuracy of QSM for brain iron mapping and also confirms ferric iron as the dominant susceptibility source in subcortical GM, by demonstrating high linear correlation of QSM to Perls' ferric iron staining.

KEYWORDS:

Brain iron; Perls' iron stain; Postmortem imaging; Quantitative susceptibility mapping; R2* mapping; Subcortical gray matter

[Indexed for MEDLINE]

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