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Curr Opin Biotechnol. 2015 Feb;31:108-16. doi: 10.1016/j.copbio.2014.10.011. Epub 2014 Nov 11.

Secondary ion mass spectrometry and Raman spectroscopy for tissue engineering applications.

Author information

1
Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, USA.
2
Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, USA; Department of Chemistry, University of Illinois at Urbana-Champaign, USA; Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, USA. Electronic address: mlkraft@illinois.edu.

Abstract

Identifying the matrix properties that permit directing stem cell fate is crucial for expanding desired cell lineages ex vivo for disease treatment. Such efforts require knowledge of matrix surface chemistry and the cell responses they elicit. Recent progress in analyzing biomaterial composition and identifying cell phenotype with two label-free chemical imaging techniques, TOF-SIMS and Raman spectroscopy are presented. TOF-SIMS is becoming indispensable for the surface characterization of biomaterial scaffolds. Developments in TOF-SIMS data analysis enable correlating surface chemistry with biological response. Advances in the interpretation of Raman spectra permit identifying the fate decisions of individual, living cells with location specificity. Here we highlight this progress and discuss further improvements that would facilitate efforts to develop artificial scaffolds for tissue regeneration.

PMID:
25462628
PMCID:
PMC4297681
DOI:
10.1016/j.copbio.2014.10.011
[Indexed for MEDLINE]
Free PMC Article

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