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Fish Shellfish Immunol. 2015 Jan;42(1):213-20. doi: 10.1016/j.fsi.2014.11.004. Epub 2014 Nov 14.

Single-walled carbon nanotubes as delivery vehicles enhance the immunoprotective effects of a recombinant vaccine against Aeromonas hydrophila.

Author information

1
Northwest A&F University, Yangling, 712100, People's Republic of China.
2
Northwest A&F University, Yangling, 712100, People's Republic of China. Electronic address: wanggaoxue@126.com.
3
Northwest A&F University, Yangling, 712100, People's Republic of China. Electronic address: 286567031@qq.com.

Abstract

To reduce the economic losses caused by diseases in aquaculture industry, more efficient and economic prophylactic measures should be urgently investigated. In this research, the effects of a novel functionalized single-walled carbon nanotubes (SWCNTs) applied as a delivery vehicle for recombinant Aeromonas hydrophila vaccine administration via bath or injection in juvenile grass carp were studied. The results showed that SWCNT as a vector for the recombinant protein aerA, augmented the production of specific antibodies, apparently stimulated the induction of immune-related genes, and induced higher level of survival rate compared with free aerA subunit vaccine. Furthermore, we compared the routes of bath and intramuscular injection immunization by SWCNTs-aerA vaccine, and found that similar antibody levels induced by SWCNTs-aerA were observed in both immunization routes. Meanwhile, a similar relative percentage survival (approximately 80%) was found in both a 40 mg/L bath immunization group, and a 20 μg injection group. The results indicate that functionalized SWCNTs could be a promising delivery vehicle to potentiate the immune response of recombinant vaccines, and might be used to vaccinate juvenile fish by bath administration method.

KEYWORDS:

Aeromonas hydrophila; Bath immunization; Immune response; Single-walled carbon nanotubes; Vaccine

PMID:
25462556
DOI:
10.1016/j.fsi.2014.11.004
[Indexed for MEDLINE]

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