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Virology. 2015 Jan 15;475:88-95. doi: 10.1016/j.virol.2014.11.008. Epub 2014 Nov 27.

An MHC class I immune evasion gene of Marek׳s disease virus.

Author information

1
Department of Comparative Medicine & Integrative Biology, Michigan State University, East Lansing, MI, USA.
2
Department of Microbiology & Molecular Genetics, Michigan State University, East Lansing, MI, USA.
3
Department of Microbiology & Molecular Genetics, Michigan State University, East Lansing, MI, USA; United States Department of Agriculture, Agriculture Research Service, Avian Disease and Oncology Laboratory, 4279 East Mount Hope Road, East Lansing, MI 48823, USA.
4
Department of Microbiology & Molecular Genetics, Michigan State University, East Lansing, MI, USA. Electronic address: ite1@cdc.gov.

Abstract

Marek׳s disease virus (MDV) is a widespread α-herpesvirus of chickens that causes T cell tumors. Acute, but not latent, MDV infection has previously been shown to lead to downregulation of cell-surface MHC class I (Virology 282:198-205 (2001)), but the gene(s) involved have not been identified. Here we demonstrate that an MDV gene, MDV012, is capable of reducing surface expression of MHC class I on chicken cells. Co-expression of an MHC class I-binding peptide targeted to the endoplasmic reticulum (bypassing the requirement for the TAP peptide transporter) partially rescued MHC class I expression in the presence of MDV012, suggesting that MDV012 is a TAP-blocking MHC class I immune evasion protein. This is the first unique non-mammalian MHC class I immune evasion gene identified, and suggests that α-herpesviruses have conserved this function for at least 100 million years.

KEYWORDS:

Immune evasion; Major histocompatibility complex class I; Marek׳s disease virus

PMID:
25462349
PMCID:
PMC4280340
DOI:
10.1016/j.virol.2014.11.008
[Indexed for MEDLINE]
Free PMC Article

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