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Eur J Obstet Gynecol Reprod Biol. 2015 Jan;184:1-6. doi: 10.1016/j.ejogrb.2014.10.041. Epub 2014 Nov 11.

Is resveratrol a potential substitute for leuprolide acetate in experimental endometriosis?

Author information

1
Departments of Gynecology and Obstetrics, Recep Tayyip Erdogan University, School of Medicine, 53020 Rize, Turkey. Electronic address: yesimbay@yahoo.com.
2
Department of Obstetrics and Gynecology, School of Medicine, Black Sea Technical University, 61080 Trabzon, Turkey.
3
Departments of Biochemistry, Recep Tayyip Erdogan University, School of Medicine, 53020 Rize, Turkey.
4
Departments of Histology, Recep Tayyip Erdogan University, School of Medicine, 53020 Rize, Turkey.
5
Departments of Gynecology and Obstetrics, Recep Tayyip Erdogan University, School of Medicine, 53020 Rize, Turkey.

Abstract

OBJECTIVE:

Resveratrol, a phytoalexin polyphenol, has anti-angiogenic, antioxidant, anti-inflammatory properties. We aimed to compare the anti-inflammatory and anti-angiogenic effects of resveratrol and leuprolide acetate (LA) in an experimental endometriosis model.

STUDY DESIGN:

A prospective experimental study was conducted in a University Surgical Research Center. Thirty-three non-pregnant female Sprague-Dawley rats, in which experimental model of endometriosis were surgically induced were randomly divided into four groups. Group 1 was administered 30 mg/kg resveratrol i.m. for 14 days, group 2 was given 1mg/kg s.c. single dose LA, group 3 was administered both resveratrol and LA, and group 4 had no medication. After two weeks medication rats were sacrificed and size, histopathology and immunreactivity to matrix metalloproteinase (mmp)2, mmp9, vascular endothelial growth factor (VEGF) of the endometriotic implants were evaluated. Plasma and peritoneal fluid levels of interleukin (IL)-6, IL-8, and tumor necrosis factor-α (TNF-α) were analyzed.

RESULTS:

The endometriotic implant volumes, histopathological grade and immunreactivity to mmp2, mmp9 and VEGF were significantly reduced (p<0.001), and plasma and peritoneal fluid levels of IL-6, IL-8 and TNF-α were significantly decreased in group 1 and group 2 in comparison to group 3 and group 4 (p < 0.001).

CONCLUSION:

Resveratrol alone is a potential agent for the treatment of endometriosis and may be an alternative to LA. In contrast, the combination of LA and resveratrol decreased the anti-inflammatory and anti-angiogenic effects of each agent. Since resveratrol is widely used as an alternative therapy for a variety of conditions, it can undermine the effectiveness of LA. Therefore, caution should be exercised when used in combination with other agents.

KEYWORDS:

Endometriosis; Leuprolide acetate; Rat model; Resveratrol; Treatment

PMID:
25462211
DOI:
10.1016/j.ejogrb.2014.10.041
[Indexed for MEDLINE]

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