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J Microbiol Methods. 2015 Jan;108:36-44. doi: 10.1016/j.mimet.2014.11.007. Epub 2014 Nov 20.

Simulating distal gut mucosal and luminal communities using packed-column biofilm reactors and an in vitro chemostat model.

Author information

1
Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada.
2
Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.
3
Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada. Electronic address: eav@uoguelph.ca.

Abstract

In vivo studies of human mucosal gut microbiota are often limited to end-point analyses and confounded by bowel cleansing procedures. Therefore, we used biofilm reactors to incorporate a simulated mucosal environment into an in vitro gut chemostat model. Communities developed were complex, reproducible, distinct, and representative of in vivo communities.

KEYWORDS:

Biofilm; Chemostat; DGGE; HITChip; Human gut microbiota; Microbial ecology

PMID:
25462016
DOI:
10.1016/j.mimet.2014.11.007
[Indexed for MEDLINE]

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