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Metab Eng. 2015 Jan;27:76-82. doi: 10.1016/j.ymben.2014.11.001. Epub 2014 Nov 15.

Potential production platform of n-butanol in Escherichia coli.

Author information

1
Department of Chemical Engineering, Feng Chia University, 100 Wenhwa Road, Taichung 40724, Taiwan.
2
Department of Medical Laboratory Science and Biotechnology, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan. Electronic address: oleosin91@yahoo.com.tw.
3
Department of Chemical Engineering, Feng Chia University, 100 Wenhwa Road, Taichung 40724, Taiwan; Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan; Department of Medical Research, China Medical University Hospital, Taichung 40447, Taiwan. Electronic address: ypchao@fcu.edu.tw.

Abstract

We proposed a potential production platform of n-butanol in Escherichia coli. First, a butyrate-conversion strain was developed by removal of undesired genes and recruiting endogenous atoDA and Clostridium adhE2. Consequently, this E. coli strain grown on the M9 mineral salt with yeast extract (M9Y) was shown to produce 6.2g/L n-butanol from supplemented butyrate at 36h. The molar conversion yield of n-butanol on butyrate reaches 92%. Moreover, the production platform was advanced by additional inclusion of a butyrate-producing strain. This strain was equipped with a pathway comprising atoDA and heterologous genes for the synthesis of butyrate. Without butyrate, the butyrate-conversion and the butyrate-producing strains were co-cultured in M9Y medium and produced 5.5g/L n-butanol from glucose at 24h. The production yield on glucose accounts for 69% of the theoretical yield. Overall, it indicates a promise of the developed platform for n-butanol production in E. coli.

KEYWORDS:

Butyrate; Co-culturing; Metabolic engineering; n-Butanol

PMID:
25461833
DOI:
10.1016/j.ymben.2014.11.001
[Indexed for MEDLINE]

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