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Virology. 2014 Dec;471-473:126-40. doi: 10.1016/j.virol.2014.10.008. Epub 2014 Oct 31.

Discovery and biological characterization of two novel pig-tailed macaque homologs of HHV-6 and HHV-7.

Author information

1
Center for Global Infectious Disease Research, Seattle Children׳s Research Institute, Seattle, Research Center, Seattle, WA, USA. Electronic address: jstaheli@seattlechildrens.org.
2
Center for Global Infectious Disease Research, Seattle Children׳s Research Institute, Seattle, Research Center, Seattle, WA, USA. Electronic address: michael.dyen@seattlechildrens.org.
3
Center for Global Infectious Disease Research, Seattle Children׳s Research Institute, Seattle, Research Center, Seattle, WA, USA. Electronic address: patrick.lewis@seattlechildrens.org.
4
Center for Global Infectious Disease Research, Seattle Children׳s Research Institute, Seattle, Research Center, Seattle, WA, USA; Department of Pediatrics, University of Washington, Seattle, WA, USA. Electronic address: serge.barcy@seattlechildrens.org.

Abstract

Human herpesvirus-6 (HHV-6) and -7 (HHV-7) are Roseoloviruses within the Betaherpesvirus family, which have a high prevalence and suspected involvement in a number of diseases. Using CODEHOP-based PCR, we identified homologs of both viruses in saliva of pig-tailed macaques, provisionally named MneHV-6 and MneHV-7. This finding supports the existence of two distinct Roseolovirus lineages before the divergence of humans and macaques. Using specific qPCR assays, high levels of MneHV-6 and MneHV-7 DNA were detected in macaque saliva, although the frequency was greater for MneHV-7. A blood screen of 283 macaques revealed 10% MneHV-6 DNA positivity and 25% MneHV-7 positivity, with higher prevalences of MneHV-6 in older females and of MneHV-7 in younger males. Levels of MneHV-6 were increased in animals coinfected with MneHV-7, and both viruses were frequently detected in salivary gland and stomach tissues. Our discovery provides a unique animal model to answer unresolved questions regarding Roseolovirus pathology.

KEYWORDS:

CODEHOP; Human herpesvirus-6; Human herpesvirus-7; Macaca nemestrina; Oral swab; Prevalence; Roseoloviruses; Saliva; Tropism

PMID:
25461538
PMCID:
PMC4312206
DOI:
10.1016/j.virol.2014.10.008
[Indexed for MEDLINE]
Free PMC Article

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