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Biosens Bioelectron. 2015 Mar 15;65:211-9. doi: 10.1016/j.bios.2014.10.036. Epub 2014 Oct 22.

A novel immunochromatographic system for easy-to-use detection of group 1 avian influenza viruses with acquired human-type receptor binding specificity.

Author information

1
Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan. Electronic address: nabe@biken.osaka-u.ac.jp.
2
KAINOS Laboratories, Inc., Tokyo 113-0033, Japan.
3
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt.
4
Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan.
5
Center for Infectious Disease Research in Asia and Africa, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan; Vietnam Research Station, Nagasaki University, c/o National Institute of Hygiene and Epidemiology, Hanoi, Viet Nam.
6
Department of Virology, National Institute of Hygiene and Epidemiology, Hanoi, Viet Nam.
7
Avian Zoonosis Research Center, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
8
Center for Infectious Diseases, Graduate School of Medicine, Kobe University, Hyogo 650-0017, Japan.
9
Indonesia-Japan Collaborative Research Center, Institute of Tropical Disease, Airlangga University, Surabaya 60115, Indonesia.
10
Health Science Hills, College of Life and Health Sciences, Chubu University, Aichi 487-8501, Japan.
11
Department of Infectious Diseases, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
12
Kanonji Institute, The Research Foundation for Microbial Diseases of Osaka University, Kagawa 768-0061, Japan.
13
Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani, 12120, Thailand; Health Science Hills, College of Life and Health Sciences, Chubu University, Aichi 487-8501, Japan.
14
Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526, Japan.
15
Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan.
16
Health Science Hills, College of Life and Health Sciences, Chubu University, Aichi 487-8501, Japan. Electronic address: suzukiy@isc.chubu.ac.jp.

Abstract

A switch of viral hemagglutinin receptor binding specificity from bird-type α2,3- to human-type α2,6-linked sialic acid is necessary for an avian influenza virus to become a pandemic virus. In this study, an easy-to-use strip test to detect receptor binding specificity of influenza virus was developed. A biotinylated anti-hemagglutinin antibody that bound a broad range of group 1 influenza A viruses and latex-conjugated α2,3 (blue) and α2,6 (red) sialylglycopolymers were used in an immunochromatographic strip test, with avidin and lectin immobilized on a nitrocellulose membrane at test and control lines, respectively. Accumulation of a sialylglycopolymer-virus-antibody complex at the test line was visualized by eye. The strip test could be completed in 30min and did not require special equipment or skills, thereby avoiding some disadvantages of current methods for analyzing receptor binding specificity of influenza virus. The strip test could detect the receptor binding specificity of a wide range of influenza viruses, as well as small increases in the binding affinity of variant H5N1 viruses to α2,6 sialylglycans at viral titers >128 hemagglutination units. The strip test results were in agreement with those of ELISA virus binding assays, with correlations >0.95. In conclusion, the immunochromatographic strip test developed in this study should be useful for monitoring potential changes in the receptor binding specificity of group 1 influenza A viruses in the field.

KEYWORDS:

Detection of receptor binding specificity; Group 1 influenza A virus; H5N1 avian influenza virus; Immunochromatographic strip test; Pandemic potential; Sialylglycopolymer

PMID:
25461160
DOI:
10.1016/j.bios.2014.10.036
[Indexed for MEDLINE]

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