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Neurobiol Learn Mem. 2015 Feb;118:80-8. doi: 10.1016/j.nlm.2014.11.010. Epub 2014 Nov 22.

Spatio-temporal in vivo recording of dCREB2 dynamics in Drosophila long-term memory processing.

Author information

1
Neuroscience Training Program, 1300 University Ave., University of Wisconsin-Madison, Madison, WI 53706, United States; Department of Genetics, 3434 Genetics/Biotechnology, 425 Henry Mall, University of Wisconsin-Madison, Madison, WI 53706, United States. Electronic address: jzhang84@wisc.edu.
2
Neuroscience Training Program, 1300 University Ave., University of Wisconsin-Madison, Madison, WI 53706, United States; Department of Genetics, 3434 Genetics/Biotechnology, 425 Henry Mall, University of Wisconsin-Madison, Madison, WI 53706, United States. Electronic address: tanenhaus@wisc.edu.
3
Department of Statistics, University of Wisconsin-Madison, Madison, WI 53706, United States. Electronic address: jcdavis5@wisc.edu.
4
Department of Statistics, University of Wisconsin-Madison, Madison, WI 53706, United States. Electronic address: hanlon@stat.wisc.edu.
5
Department of Genetics, 3434 Genetics/Biotechnology, 425 Henry Mall, University of Wisconsin-Madison, Madison, WI 53706, United States; Department of Neurology, 1685 Highland Ave., University of Wisconsin-Madison, Madison, WI 53706, United States. Electronic address: jcyin@wisc.edu.

Abstract

CREB (cAMP response element-binding protein) is an evolutionarily conserved transcription factor, playing key roles in synaptic plasticity, intrinsic excitability and long-term memory (LTM) formation. The Drosophila homologue of mammalian CREB, dCREB2, is also important for LTM. However, the spatio-temporal nature of dCREB2 activity during memory consolidation is poorly understood. Using an in vivo reporter system, we examined dCREB2 activity continuously in specific brain regions during LTM processing. Two brain regions that have been shown to be important for Drosophila LTM are the ellipsoid body (EB) and the mushroom body (MB). We found that dCREB2 reporter activity is persistently elevated in EB R2/R4m neurons, but not neighboring R3/R4d neurons, following LTM-inducing training. In multiple subsets of MB neurons, dCREB2 reporter activity is suppressed immediately following LTM-specific training, and elevated during late windows. In addition, we observed heterogeneous responses across different subsets of neurons in MB αβ lobe during LTM processing. All of these changes suggest that dCREB2 functions in both the EB and MB for LTM formation, and that this activity contributes to the process of systems consolidation.

KEYWORDS:

Ellipsoid body; In vivo transcriptional activity; Long-term memory; Mushroom body; Spatio-temporal recording; cAMP response element-binding protein

PMID:
25460038
PMCID:
PMC4331215
DOI:
10.1016/j.nlm.2014.11.010
[Indexed for MEDLINE]
Free PMC Article

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