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Bioresour Technol. 2015 Jan;175:82-90. doi: 10.1016/j.biortech.2014.10.061. Epub 2014 Oct 18.

Hydrogen production by Rhodobacter sphaeroides DSM 158 under intense irradiation.

Author information

1
Institute of Food Technology and Bioprocess Engineering, TU Dresden, Bergstraße 120, 01069 Dresden, Germany. Electronic address: Felix.Krujatz@tu-dresden.de.
2
Institute of Food Technology and Bioprocess Engineering, TU Dresden, Bergstraße 120, 01069 Dresden, Germany. Electronic address: paulhaertel@gmx.de.
3
Institute of Food Technology and Bioprocess Engineering, TU Dresden, Bergstraße 120, 01069 Dresden, Germany. Electronic address: Karsten.Helbig@tu-dresden.de.
4
Institute of Physical Chemistry, TU Dresden, Eisenstuckstraße 5, 01069 Dresden, Germany. Electronic address: Nora.Haufe@tu-dresden.de.
5
Institute of Food Technology and Bioprocess Engineering, TU Dresden, Bergstraße 120, 01069 Dresden, Germany. Electronic address: Simone.Thierfelder@tu-dresden.de.
6
Institute of Food Technology and Bioprocess Engineering, TU Dresden, Bergstraße 120, 01069 Dresden, Germany. Electronic address: Thomas.Bley@tu-dresden.de.
7
Institute of Food Technology and Bioprocess Engineering, TU Dresden, Bergstraße 120, 01069 Dresden, Germany. Electronic address: Jost.Weber@tu-dresden.de.

Abstract

To identify optimal hydrogen production conditions using growing cultures of Rhodobacter sphaeroides DSM 158 the effects of varying the reactor's volumetric power input (0.01-1.4kWm(-3)) and irradiation intensity (5-2500Wm(-2)) were investigated in batch and continuous production modes. Irradiation intensity had a greater effect on hydrogen production than volumetric power input. Hydrogen production and photofermentative biomass formation were maximized by irradiation at 2250Wm(-2) with a volumetric power input of 0.55kWm(-3). The bacterial dry weight (2.64gL(-1)) and rate of hydrogen production (195mLL(-1)h(-1)) achieved under these conditions were greater than any that have previously been reported for batch-mode hydrogen production by R. sphaeroides. Continuous mode experiments (D=0.1h(-1)) yielded a bacterial dry weight, hydrogen production rate, productivity and hydrogen yield of 2.35±0.18gL(-1), 165±6.2mLL(-1)h(-1), 3.96LL(-1)d(-1) and 36.6%, respectively.

KEYWORDS:

Batch; Continuous; Hydrogen; Intense irradiation; Photofermentation

PMID:
25459807
DOI:
10.1016/j.biortech.2014.10.061
[Indexed for MEDLINE]

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