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Virus Res. 2015 Jan 2;195:172-6. doi: 10.1016/j.virusres.2014.10.014. Epub 2014 Oct 23.

The haemagglutination activity of equine herpesvirus type 1 glycoprotein C.

Author information

1
Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
2
Molecular Biology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi 329-0412, Japan.
3
Department of Veterinary Virology, Rakuno Gakuen University, Ebetsu, Hokkaido 069-0836, Japan.
4
Laboratory of Emerging Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
5
Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan. Electronic address: kmaeda@yamaguchi-u.ac.jp.

Abstract

Equine herpesvirus type 1 (EHV-1) has haemagglutination (HA) activity toward equine red blood cells (RBCs), but the identity of its haemagglutinin is unknown. To identify the haemagglutinin of EHV-1, the major glycoproteins of EHV-1 were expressed in 293T cells, and the cells or cell lysates were mixed with equine RBCs. The results showed that only EHV-1 glycoprotein C (gC)-producing cells adsorbed equine RBCs, and that the lysate of EHV-1 gC-expressing cells agglutinated equine RBCs. EHV-1 lacking gC did not show HA activity. HA activity was inhibited by monoclonal antibodies (MAbs) specific for gC, but not by antibodies directed against other glycoproteins. In addition, HA activity was not inhibited by the addition of heparin. These results indicate that EHV-1 gC can bind equine RBCs irrespective of heparin, in contrast to other herpesvirus gC proteins.

KEYWORDS:

Equine herpesvirus type 1; Glycoprotein C; Haemagglutination activity; Heparin

PMID:
25456403
DOI:
10.1016/j.virusres.2014.10.014
[Indexed for MEDLINE]

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