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FEBS Lett. 2015 Jan 2;589(1):37-44. doi: 10.1016/j.febslet.2014.11.021. Epub 2014 Nov 29.

Glutathionyl-hydroquinone reductases from poplar are plastidial proteins that deglutathionylate both reduced and oxidized glutathionylated quinones.

Author information

1
Université de Lorraine, Interactions Arbres - Microorganismes, UMR1136, F-54500 Vandœuvre-lès-Nancy, France; INRA, Interactions Arbres - Microorganismes, UMR1136, F-54280 Champenoux, France.
2
Institut de Biologie Moléculaire des Plantes, CNRS-UPR 2357, 67084 Strasbourg, France.
3
Laboratoire d'Etudes et de Recherches sur le Matériau Bois, EA 1093, Vandœuvre-lès-Nancy F-54506, France.
4
Université de Lorraine, CRM(2), Equipe BioMod, UMR 7036, Faculté des Sciences et Technologies, BP 70239, 54506 Vandœuvre-lès-Nancy, France; CNRS, CRM(2), Equipe BioMod, UMR 7036, Faculté des Sciences et Technologies, BP 70239, 54506 Vandœuvre-lès-Nancy, France.
5
Institut Pasteur, Plate-Forme de Cristallographie, CNRS-UMR3528, 75724 Paris, France.
6
Université de Lorraine, Interactions Arbres - Microorganismes, UMR1136, F-54500 Vandœuvre-lès-Nancy, France; INRA, Interactions Arbres - Microorganismes, UMR1136, F-54280 Champenoux, France. Electronic address: arnaud.hecker@univ-lorraine.fr.

Abstract

Glutathionyl-hydroquinone reductases (GHRs) catalyze the deglutathionylation of quinones via a catalytic cysteine. The two GHR genes in the Populus trichocarpa genome, Pt-GHR1 and Pt-GHR2, are primarily expressed in reproductive organs. Both proteins are localized in plastids. More specifically, Pt-GHR2 localizes in nucleoids. At the structural level, Pt-GHR1 adopts a typical GHR fold, with a dimerization interface comparable to that of the bacterial and fungal GHR counterparts. Pt-GHR1 catalyzes the deglutathionylation of both reduced and oxidized glutathionylated quinones, but the enzyme is more catalytically efficient with the reduced forms.

KEYWORDS:

Deglutathionylation; Glutathione transferase; Plastid; Poplar

PMID:
25455804
DOI:
10.1016/j.febslet.2014.11.021
[Indexed for MEDLINE]
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