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Neuron. 2014 Nov 19;84(4):697-707. doi: 10.1016/j.neuron.2014.10.011. Epub 2014 Nov 6.

Direct targeted quantitative molecular imaging of neurotransmitters in brain tissue sections.

Author information

1
Biomolecular Imaging and Proteomics, National Center for Mass Spectrometry Imaging, Department of Pharmaceutical Biosciences, Uppsala University, P.O. Box 591 BMC, 75124 Uppsala, Sweden.
2
Biomolecular Imaging and Proteomics, National Center for Mass Spectrometry Imaging, Department of Pharmaceutical Biosciences, Uppsala University, P.O. Box 591 BMC, 75124 Uppsala, Sweden; AstraZeneca R&D, Alderley Park, Macclesfield, Cheshire SK10 4TF, UK.
3
Center for Molecular Medicine, Department of Neurology and Clinical Neuroscience, Karolinska Institutet and Karolinska University Hospital, 17176 Stockholm, Sweden.
4
Faculty of Life Sciences, University of Manchester, Manchester M13 9PL, UK.
5
Université de Bordeaux, Institut des Maladies Neurodégénératives, UMR 5293 Bordeaux, France.
6
Biomolecular Imaging and Proteomics, National Center for Mass Spectrometry Imaging, Department of Pharmaceutical Biosciences, Uppsala University, P.O. Box 591 BMC, 75124 Uppsala, Sweden. Electronic address: per.andren@farmbio.uu.se.

Abstract

Current neuroimaging techniques have very limited abilities to directly identify and quantify neurotransmitters from brain sections. We have developed a molecular-specific approach for the simultaneous imaging and quantitation of multiple neurotransmitters, precursors, and metabolites, such as tyrosine, tryptamine, tyramine, phenethylamine, dopamine, 3-methoxytyramine, serotonin, GABA, glutamate, acetylcholine, and L-alpha-glycerylphosphorylcholine, in histological tissue sections at high spatial resolutions. The method is employed to directly measure changes in the absolute and relative levels of neurotransmitters in specific brain structures in animal disease models and in response to drug treatments, demonstrating the power of mass spectrometry imaging in neuroscience.

PMID:
25453841
DOI:
10.1016/j.neuron.2014.10.011
[Indexed for MEDLINE]
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