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Mol Cell. 2014 Dec 18;56(6):763-76. doi: 10.1016/j.molcel.2014.10.015. Epub 2014 Nov 20.

Expression of nuclear and mitochondrial genes encoding ATP synthase is synchronized by disassembly of a multisynthetase complex.

Author information

1
Génétique Moléculaire, Génomique, Microbiologie, UMR 7156, CNRS, Université de Strasbourg, 21 Rue René Descartes, 67084 Strasbourg Cedex, France; UPR Architecture et Réactivité de l'ARN, Institut de Biologie Moléculaire et Cellulaire, Université de Strasbourg, CNRS, 15 Rue René Descartes, 67084 Strasbourg Cedex, France. Electronic address: mathieu.frechin@uzh.ch.
2
Génétique Moléculaire, Génomique, Microbiologie, UMR 7156, CNRS, Université de Strasbourg, 21 Rue René Descartes, 67084 Strasbourg Cedex, France.
3
Centre National de la Recherche Scientifique, Institut de Biochimie et Génétique Cellulaires, UMR 5095, 1 Rue Camille Saint-Saëns, 33077 Bordeaux, France; Institut de Biochimie et Génétique Cellulaires, UMR 5095, Université Bordeaux, 146 Rue Léo Saignat, 33076 Bordeaux, France.
4
Plateforme Protéomique de l'Esplanade, Université de Strasbourg, CNRS- FRC1589, IBMC, 15 Rue René Descartes, 67084 Strasbourg Cedex, France.
5
European Molecular Biology Laboratory (EMBL), Genome Biology Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
6
European Molecular Biology Laboratory (EMBL), Genome Biology Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany; Stanford Genome Technology Center, Stanford University, Palo Alto, CA 94304, USA and Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.
7
Centre National de la Recherche Scientifique, Institut de Biochimie et Génétique Cellulaires, UMR 5095, 1 Rue Camille Saint-Saëns, 33077 Bordeaux, France; Institut de Biochimie et Génétique Cellulaires, UMR 5095, Université Bordeaux, 146 Rue Léo Saignat, 33076 Bordeaux, France. Electronic address: jp.dirago@ibgc.cnrs.fr.
8
Génétique Moléculaire, Génomique, Microbiologie, UMR 7156, CNRS, Université de Strasbourg, 21 Rue René Descartes, 67084 Strasbourg Cedex, France. Electronic address: h.becker@unistra.fr.

Abstract

In eukaryotic cells, oxidative phosphorylation involves multisubunit complexes of mixed genetic origin. Assembling these complexes requires an organelle-independent synchronizing system for the proper expression of nuclear and mitochondrial genes. Here we show that proper expression of the F1FO ATP synthase (complex V) depends on a cytosolic complex (AME) made of two aminoacyl-tRNA synthetases (cERS and cMRS) attached to an anchor protein, Arc1p. When yeast cells adapt to respiration the Snf1/4 glucose-sensing pathway inhibits ARC1 expression triggering simultaneous release of cERS and cMRS. Free cMRS and cERS relocate to the nucleus and mitochondria, respectively, to synchronize nuclear transcription and mitochondrial translation of ATP synthase genes. Strains releasing asynchronously the two aminoacyl-tRNA synthetases display aberrant expression of nuclear and mitochondrial genes encoding subunits of complex V resulting in severe defects of the oxidative phosphorylation mechanism. This work shows that the AME complex coordinates expression of enzymes that require intergenomic control.

PMID:
25453761
DOI:
10.1016/j.molcel.2014.10.015
[Indexed for MEDLINE]
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