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Genes Dev. 2014 Dec 1;28(23):2591-6. doi: 10.1101/gad.248864.114.

Differential spatial and structural organization of the X chromosome underlies dosage compensation in C. elegans.

Author information

1
Cell Fate and Nuclear Organization, Institute of Cell Biology, University of Bern, 3012 Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences, University of Bern, 3012 Bern, Switzerland;
2
Laboratoire de Physique, Ecole Normale Supérieure de Lyon, UMR 5672, Centre National de la Recherche Scientifique (CNRS), 69007 Lyon, France;
3
Division of Gene Regulation, The Netherlands Cancer Institute, 1006 Amsterdam, The Netherlands;
4
Spanish National Research Council (CSIC).
5
Spanish National Research Council (CSIC), The Junta of Andalusia (JA), Universidad Pablo de Olavide, Andalusian Center for Developmental Biology (CABD), 41013 Sevilla, Spain.
6
Cell Fate and Nuclear Organization, Institute of Cell Biology, University of Bern, 3012 Bern, Switzerland; peter.meister@izb.unibe.ch.

Abstract

The adjustment of X-linked gene expression to the X chromosome copy number (dosage compensation [DC]) has been widely studied as a model of chromosome-wide gene regulation. In Caenorhabditis elegans, DC is achieved by twofold down-regulation of gene expression from both Xs in hermaphrodites. We show that in males, the single X chromosome interacts with nuclear pore proteins, while in hermaphrodites, the DC complex (DCC) impairs this interaction and alters X localization. Our results put forward a structural model of DC in which X-specific sequences locate the X chromosome in transcriptionally active domains in males, while the DCC prevents this in hermaphrodites.

KEYWORDS:

C. elegans; dosage compensation; nuclear organization; nuclear pores

PMID:
25452271
PMCID:
PMC4248290
DOI:
10.1101/gad.248864.114
[Indexed for MEDLINE]
Free PMC Article

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