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Diabetes Res Clin Pract. 2014 Dec;106(3):634-42. doi: 10.1016/j.diabres.2014.09.022. Epub 2014 Oct 5.

Effect of maternal use of flaxseed oil during pregnancy and lactation on glucose metabolism and pancreas histomorphometry of male offspring from diabetic rats.

Author information

1
Laboratory of Experimental Nutrition, Department of Nutrition and Dietetics, Nutrition College, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil. Electronic address: andremcorreia@gmail.com.
2
Laboratory of Experimental Nutrition, Department of Nutrition and Dietetics, Nutrition College, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil.
3
Medical College, Rio de Janeiro Feredal University - Campus UFRJ, Macaé, Rio de Janeiro, Brazil.

Abstract

AIM:

Investigate if the maternal use of flaxseed oil prevents pancreatic alterations in the offspring of diabetic mothers.

METHODS:

Diabetes was induced in female wistar rats (n=12) by a high-fat diet and low-dose of streptozotocin. After the confirmation of the diabetes (glucose >300 mg/dL), rats were mated and once pregnancy was confirmed, they were allocated into three groups (n=6): high-fat group (HFG); flaxseed oil group (FOG); and control group (CG) (nondiabetic rats). At weaning, male offspring (n=12/group) received a standard chow diet. The animals were euthanized in two phases: at 100 and at 180 days, (n=6/group). The pancreas was collected for histomorphometric and immunohistochemistry analysis.

RESULTS:

HFG showed hypertrophy of pancreatic islets at 100 and at 180 days (p<0.0001), while the FOG offspring had islets with smaller diameters compared to HFG at both phases of sacrifice (p<0.0001). HFG had a lower percentage of small islets when compared to CG and FOG, which had a higher percentage when compared to HFG (p=0.0053) at 100 days. At 180 days HFG showed higher percentage of larger islets (p=0.00137) and lower percentage of smaller islets (p=0.00112), when compared to FOG. HFG showed lower islet insulin immunodensity at 100 days (p<0.0001) and 180 days (p<0.0001), whereas FOG was similar to CG (p<0.0001) at 100 days and higher at 180 days (p<0.0001).

CONCLUSIONS:

Flaxseed oil reduced the damage caused by maternal hyperglycemia, promoting normal pancreas histomorphometry and β cell mass.

KEYWORDS:

Diabetes; Flaxseed oil; Gestational; Metabolic programming; Pancreas; Rat

PMID:
25451892
DOI:
10.1016/j.diabres.2014.09.022
[Indexed for MEDLINE]

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