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J Microbiol Methods. 2015 Jan;108:45-8. doi: 10.1016/j.mimet.2014.11.008. Epub 2014 Nov 20.

Evaluation of a simple phenotypic method for the detection of carbapenemase-producing Enterobacteriaceae.

Author information

1
Department of Microbiology and Immunology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan. Electronic address: r-saito.mi@tmd.ac.jp.
2
Department of Infection Control and Prevention, The University of Tokyo Hospital, Tokyo, Japan. Electronic address: skoyano-tky@umin.ac.jp.
3
Department of Microbiology and Immunology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan. Electronic address: rindorindo2@gmail.com.
4
Department of Infection Control and Prevention, The University of Tokyo Hospital, Tokyo, Japan. Electronic address: yoshimi-tky@umin.ac.jp.
5
Department of Infection Control and Prevention, The University of Tokyo Hospital, Tokyo, Japan. Electronic address: misawa-tky@umin.org.
6
Medical Microbiology Laboratory, Funabashi Municipal Medical Center, Chiba, Japan. Electronic address: naganoyn@d3.dion.ne.jp.
7
Kanto Chemical Co., Inc., Tokyo, Japan. Electronic address: kaneko-takamasa@gms.kanto.co.jp.
8
Department of Infection Control and Prevention, The University of Tokyo Hospital, Tokyo, Japan. Electronic address: moriya-tky@umin.org.

Abstract

We investigated the performance of a phenotypic test, the Carbapenemase Detection Set (MAST-CDS), for the identification of carbapenemase-producing Enterobacteriaceae. Our results indicated that MAST-CDS is rapid, easily performed, simple to interpret, and highly sensitive for the identification of carbapenemase producers, particularly imipenemase producers.

KEYWORDS:

Carbapenemase; Enterobacteriaceae; Imipenemase; Klebsiella pneumoniae carbapenemase; New Delhi metallo-β-lactamase; Oxacillinase-48-like

PMID:
25451461
DOI:
10.1016/j.mimet.2014.11.008
[Indexed for MEDLINE]

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