Identification of insertion sequence element IS427 in pTiT37 plasmid DNA of an Agrobacterium tumefaciens T37 isolate

C De Meirsman; C Croes; J Desair; C Verreth; A Van Gool; J Vanderleyden

doi:10.1016/0147-619x(89)90056-5

Identification of insertion sequence element IS427 in pTiT37 plasmid DNA of an Agrobacterium tumefaciens T37 isolate

Plasmid. 1989 Mar;21(2):129-37. doi: 10.1016/0147-619x(89)90056-5.

Authors

C De Meirsman¹, C Croes, J Desair, C Verreth, A Van Gool, J Vanderleyden

Affiliation

¹ F. A. Janssens Memorial Laboratory for Genetics, University of Leuven,Heverlee, Belgium.

PMID: 2544912
DOI: 10.1016/0147-619x(89)90056-5

Abstract

The isolation and characterization of an insertion sequence (IS) element, IS427, from Agrobacterium tumefaciens T37 is described. IS427 is present in three nonidentical copies on the pTiT37 plasmid. The copy that was isolated through transposition on the entrapment vector pUCD800 contains at its ends a 16-bp imperfect inverted repeat and generates a 2-bp duplication of the target DNA. IS427 does not show homology with previously characterized IS elements of A. tumefaciens, based on hybridization experiments and/or sequence comparison.

MeSH terms

Bacillus subtilis / genetics
Base Sequence
DNA Transposable Elements*
DNA, Bacterial / genetics
DNA, Bacterial / isolation & purification
Genes, Bacterial*
Genes, Regulator*
Molecular Sequence Data
Plasmids*
Restriction Mapping
Rhizobium / genetics*

Substances

DNA Transposable Elements
DNA, Bacterial