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Toxicol In Vitro. 2015 Sep;29(6):1275-82. doi: 10.1016/j.tiv.2014.10.022. Epub 2014 Nov 4.

Lipid antigen presentation through CD1d pathway in mouse lung epithelial cells, macrophages and dendritic cells and its suppression by poly-dispersed single-walled carbon nanotubes.

Author information

1
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
2
Faculty of Life Sciences and Biotechnology, South Asian University, New Delhi, India. Electronic address: rajivksaxena@hotmail.com.

Abstract

Effect of poly-dispersed acid-functionalized single-walled carbon nanotubes (AF-SWCNTs) was examined on lipid antigen presentation through CD1d pathway on three cell lines, LA4, MHS, and JAWSII used as prototype antigen presenting cells (APCs). CD1d molecule was expressed on 80-90% MHS (prototype macrophages) and JAWSII (prototype dendritic cells) cells whereas <5% LA4 cells (lung epithelial cells, non-classical APCs) expressed CD1d. Treatment with AF-SWCNTs but not with pristine SWCNTs resulted in a significant decline in the level of CD1d mRNA as well as mRNA levels of some other intracellular proteins involved in lipid antigen presentation pathway (MTP, ApoE, prosaposin, SR-BI and LDLr). Lipid antigen presentation was assessed by first incubating the cells with a prototype lipid antigen (α-Glactosylceramide or αGC) and then staining with L363 monoclonal antibody that detects αGC bound to CD1d molecule. While 100% MHS and JAWSII cells presented αGC, only 20% LA4 cells presented the CD1d antigen. Treatment with AF-SWCNTs resulted in a 30-40% decrease in αGC antigen presentation in all three cell lines. These results show that AF-SWCNT treatment down regulated the lipid antigen presentation pathway in all three cell lines and significantly lowered the ability of these cell lines to present αGC antigen.

KEYWORDS:

CD1d; Carbon nanotubes; L363 mab; Lipid antigen presentation; Lungs; α-Glactosylceramide

PMID:
25448806
DOI:
10.1016/j.tiv.2014.10.022
[Indexed for MEDLINE]

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