Format

Send to

Choose Destination
Steroids. 2015 May;97:62-6. doi: 10.1016/j.steroids.2014.10.002. Epub 2014 Nov 1.

Synthesis and biological evaluation of nandrolone-bodipy conjugates.

Author information

1
Department of Chemistry of Natural Compounds, Institute of Chemical Technology in Prague, 166 28 Prague, Czech Republic.
2
Department of Biochemistry and Microbiology, Institute of Chemical Technology in Prague, 166 28 Prague, Czech Republic.
3
Department of Chemistry of Natural Compounds, Institute of Chemical Technology in Prague, 166 28 Prague, Czech Republic. Electronic address: Pavel.Drasar@vscht.cz.

Abstract

Here, we report synthesis and biological evaluation of fluorescent nandrolone-3-carboxymethyloxime derivatives conjugated with green-emitting bodipy dye via PEG linkers. All the newly-synthesized compounds were evaluated for their effect on cell proliferation in vitro in MCF-7, LNCaP, PC-3 and HEK 293T model cell lines using WST-1 assay. By means of live-cell fluorescence microscopy, the intracellular localization of nandrolone-bodipy conjugates was revealed in endoplasmic reticulum. Moreover, we performed competitive localization study with nonfluorescent nandrolone, metandrolone, boldenone, trenbolone, and testosterone.

KEYWORDS:

Androgens; Cytotoxicity; Fluorescent conjugates; Nandrolone

PMID:
25448362
DOI:
10.1016/j.steroids.2014.10.002
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center