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Methods. 2015 Jan 15;72:57-64. doi: 10.1016/j.ymeth.2014.10.033. Epub 2014 Nov 6.

Building the Connectivity Map of epigenetics: chromatin profiling by quantitative targeted mass spectrometry.

Author information

1
The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States.
2
Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, United States.
3
Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, United States; Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02114, United States.
4
The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States. Electronic address: jjaffe@broadinstitute.org.

Abstract

Epigenetic control of genome function is an important regulatory mechanism in diverse processes such as lineage commitment and environmental sensing, and in disease etiologies ranging from neuropsychiatric disorders to cancer. Here we report a robust, high-throughput targeted, quantitative mass spectrometry (MS) method to rapidly profile modifications of the core histones of chromatin that compose the epigenetic landscape, enabling comparisons among cells with differing genetic backgrounds, genomic perturbations, and drug treatments.

KEYWORDS:

Chromatin; Epigenetics; Histones; Mass spectrometry; Proteomics; SILAC

PMID:
25448295
PMCID:
PMC4300274
DOI:
10.1016/j.ymeth.2014.10.033
[Indexed for MEDLINE]
Free PMC Article

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