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Microb Pathog. 2014 Dec;77:73-7. doi: 10.1016/j.micpath.2014.11.002. Epub 2014 Nov 4.

Integrons in uropathogenic Escherichia coli and their relationship with phylogeny and virulence.

Author information

1
Sección Fisiología & Genética Bacterianas, Facultad de Ciencias, Iguá 4225, Montevideo 11.400, Uruguay. Electronic address: mepoey@fcien.edu.uy.
2
Sección Fisiología & Genética Bacterianas, Facultad de Ciencias, Iguá 4225, Montevideo 11.400, Uruguay. Electronic address: magela@fcien.edu.uy.

Abstract

Uropathogenic Escherichia coli (UPEC) comprise a heterogeneous group of strains. In a previous epidemiological survey performed on 230 UPEC isolates, five virulence profiles were described, each one defined by the presence of some virulence determinants and by the absence of others. Phylogenetic groups and antibiotic resistances distributed non-randomly among the isolates with different profiles. Based on these results, the presence of class 1 and 2 integrons was now investigated in these UPEC isolates in order to analyze the distribution of integrons among the phylogenetic groups and virulence profiles. As detected by PCR reactions targeted to the corresponding integrase genes, the class 1 integrons prevailed (22%) followed by those of class 2 (8%). Integrons distributed unevenly among the four main E. coli phylogenetic groups: class 1 integrons predominated in the isolates belonging to group D while class 2 were almost absent in this group. In relation to virulence, integrons frequently appeared in some virulence profiles and were particularly scarce in others. Concerning the class 1 integrons, the most notable findings were that they highly concentrated in isolates presenting one of the virulence profiles (profile V) and were absent in isolates bearing the K1 capsule. The analysis of the Pc promoter variants of the class 1 integrons revealed that all isolates with virulence profile V contained the same Pc version; PcH1. Findings in this work support the idea that, among UPEC strains, integrons would encounter constraints for their installation in some genetic backgrounds while other backgrounds would be propitious for their permanence.

KEYWORDS:

Integron; Phylogeny; Uropathogenic Escherichia coli; Virulence

PMID:
25448130
DOI:
10.1016/j.micpath.2014.11.002
[Indexed for MEDLINE]

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