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Curr Biol. 2014 Nov 17;24(22):2622-31. doi: 10.1016/j.cub.2014.09.047. Epub 2014 Oct 30.

A migrating ciliary gate compartmentalizes the site of axoneme assembly in Drosophila spermatids.

Author information

1
Department of Biological Sciences, University of Toledo, 3050 W. Towerview Boulevard, Toledo, OH 43606, USA.
2
Department of Cell Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA.
3
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Prospekt Toreza, 44, 194223 St. Petersburg, Russia.
4
Department of Biological Sciences, University of Toledo, 3050 W. Towerview Boulevard, Toledo, OH 43606, USA. Electronic address: tomer.avidorreiss@utoledo.edu.

Abstract

BACKGROUND:

In most cells, the cilium is formed within a compartment separated from the cytoplasm. Entry into the ciliary compartment is regulated by a specialized gate located at the base of the cilium in a region known as the transition zone. The transition zone is closely associated with multiple structures of the ciliary base, including the centriole, axoneme, and ciliary membrane. However, the contribution of these structures to the ciliary gate remains unclear.

RESULTS:

Here we report that, in Drosophila spermatids, a conserved module of transition zone proteins mutated in Meckel-Gruber syndrome (MKS), including Cep290, Mks1, B9d1, and B9d2, comprise a ciliary gate that continuously migrates away from the centriole to compartmentalize the growing axoneme tip. We show that Cep290 is essential for transition zone composition, compartmentalization of the axoneme tip, and axoneme integrity and find that MKS proteins also delimit a centriole-independent compartment in mouse spermatids.

CONCLUSIONS:

Our findings demonstrate that the ciliary gate can migrate away from the base of the cilium, thereby functioning independently of the centriole and of a static interaction with the axoneme to compartmentalize the site of axoneme assembly.

PMID:
25447994
PMCID:
PMC4254545
DOI:
10.1016/j.cub.2014.09.047
[Indexed for MEDLINE]
Free PMC Article
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