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Biochem Biophys Res Commun. 2015 Feb 6;457(2):125-32. doi: 10.1016/j.bbrc.2014.11.039. Epub 2014 Nov 21.

miR-320 enhances the sensitivity of human colon cancer cells to chemoradiotherapy in vitro by targeting FOXM1.

Author information

1
Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, PR China.
2
Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, PR China. Electronic address: jpxiong@medmail.com.cn.

Abstract

miR-320 expression level is found to be down-regulated in human colon cancer. To date, however, its underlying mechanisms in the chemo-resistance remain largely unknown. In this study, we demonstrated that ectopic expression of miR-320 led to inhibit HCT-116 cell proliferation, invasion and hypersensitivity to 5-Fu and Oxaliplatin. Also, knockdown of miR-320 reversed these effects in HT-29 cells. Furthermore, we identified an oncogene, FOXM1, as a direct target of miR-320. In addition, miR-320 could inactive the activity of Wnt/β-catenin pathway. Finally, we found that miR-320 and FOXM1 protein had a negative correlation in colon cancer tissues and adjacent normal tissues. These findings implied that miR-320-FOXM1 axis may overcome chemo-resistance of colon cancer cells and provide a new therapeutic target for the treatment of colon cancer.

KEYWORDS:

Chemo-resistance; Colon cancer; FOXM1; Wnt; miR-320

PMID:
25446103
DOI:
10.1016/j.bbrc.2014.11.039
[Indexed for MEDLINE]

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