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Gene. 2015 Jan 25;555(2):119-26. doi: 10.1016/j.gene.2014.10.052. Epub 2014 Oct 30.

Grape seed extract has superior beneficial effects than vitamin E on oxidative stress and apoptosis in the hippocampus of streptozotocin induced diabetic rats.

Author information

1
Izmir University School of Medicine, Department of Anatomy, Izmir, Turkey.
2
Pamukkale University School of Medicine, Department of Medical Biology, Denizli, Turkey. Electronic address: yavuzdodurga@gmail.com.
3
Pamukkale University School of Medicine, Department of Anatomy, Denizli, Turkey.
4
Pamukkale University School of Medicine, Department of Physiology, Denizli, Turkey.
5
Dokuz Eylul University School of Medicine, Department of Histology and Embryology, Izmir, Turkey.
6
Izmir Bozyaka Training and Research Hospital, Department of Pharmacology, Izmir, Turkey.
7
Mehmet Akif Ersoy University Faculty of Engineering and Architecture, Department of Food Engineering, Burdur, Turkey.

Abstract

We aimed to investigate the effects of grape seed extract (GSE) and vitamin E (Vit E) on oxidative stress and apoptosis in the hippocampus of streptozotocin-induced diabetic rats. In Control, Diabetic, and Diabetic treated with GSE (Diabetic+GSE) and vitamin E (Diabetic+Vit E) groups, oxidative stress index (OSI), TUNEL staining and Bcl-2, Bcl-XL, Bax, caspase-3, -9, and -8, Cyt-c, TNF-α, and NF-κB gene expressions were evaluated. OSI was significantly increased in the plasma and hippocampus of the Diabetic compared to Control group and decreased in Diabetic+GSE and Diabetic+Vit E groups compared to Diabetic. TUNEL positive neurons significantly increased in the hippocampus of the Diabetic group compared to Control and decreased in Diabetic+GSE (more prominently) and Diabetic+Vit E groups compared to Diabetic. In the hippocampus of the Diabetic group, Bcl-2 and Bcl-XL gene expressions were significantly decreased; Bax, caspase-3, -9, and -8, Cyt-c, TNF-α, and NF-κB gene expressions were significantly increased compared to Control. In Diabetic+GSE and Diabetic+Vit E groups, Bcl-2 gene expressions were significantly increased; Bcl-XL gene expressions did not differ compared to the Diabetic group. The expression of Bax, caspase-3, -9, and -8, Cyt-c, TNF-α, and NF-κB genes in the Diabetic+GSE group and the expression of caspase-3 and -9, TNF-α, and NF-κB genes in the Diabetic+Vit E group were significantly decreased compared to Diabetic. In conclusion, GSE (more prominently) and vitamin E decreased oxidative stress and neuronal apoptosis occurring in the hippocampus of diabetic rats.

KEYWORDS:

Apoptosis; Diabetes; Grape seed extract; Hippocampus; Oxidative stress; Vitamin E

PMID:
25445279
DOI:
10.1016/j.gene.2014.10.052
[Indexed for MEDLINE]

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