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Rev Argent Microbiol. 2014 Jul-Sep;46(3):205-9. doi: 10.1016/S0325-7541(14)70074-4. Epub 2014 Oct 15.

Diagnosis of an outbreak of Salmonella typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis.

Author information

1
Cátedra de Patología de Aves y Pilíferos y Laboratorio de Diagnóstico de Enfermedades de las Aves y los Pilíferos, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina. Electronic address: danielgornatti@fcv.unlp.edu.ar.
2
Cátedra de Microbiología y Laboratorio de Diagnóstico e Investigaciones Bacteriológicas, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
3
Cátedra de Patología de Aves y Pilíferos y Laboratorio de Diagnóstico de Enfermedades de las Aves y los Pilíferos, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
4
Servicio de Enterobacterias, Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas, ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.

Abstract

Adult chinchillas (Chinchilla lanigera) that had suddenly died in a commercial farm located in La Plata City, Buenos Aires Province, Argentina, in July 2012 were macroscopically, histopathologically, and microbiologically examined. Salmonella enterica serovar Typhimurium (S. Typhimurium) was isolated from the liver, spleen, heart, lungs, kidneys and intestines from each of the five animals evaluated. The five strains were susceptible to ampicillin, cephalotin, cefotaxime, nalidixic acid, gentamicin, streptomycin, chloramphenicol, fosfomycin, nitrofurantoin and trimethoprim-sulfamethoxazole, and resistant to tetracycline. Each of the five S. Typhimurium isolates was analyzed by XbaI- pulsed-field gel electrophoresis (PFGE), showing an identical electrophoretic profile with 15 defined bands, which was found to be identical to pattern ARJPXX01.0220 of the PulseNet Argentine National database of Salmonella PFGE patterns. This is the first work describing the postmortem diagnosis of an outbreak of salmonellosis in chinchillas by using molecular methods such as PFGE.

KEYWORDS:

Chinchilla lanigera; Chinchillas de granja; Farmed chinchillas; PFGE; Salmonella Typhimurium; Zoonosis

PMID:
25444129
DOI:
10.1016/S0325-7541(14)70074-4
[Indexed for MEDLINE]
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