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Curr Opin Biotechnol. 2015 Apr;32:76-84. doi: 10.1016/j.copbio.2014.11.007. Epub 2014 Nov 29.

Editing plant genomes with CRISPR/Cas9.

Author information

1
The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK.
2
The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address: sophien.kamoun@tsl.ac.uk.
3
The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address: nicola.patron@tsl.ac.uk.
4
The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address: vladimir.nekrasov@tsl.ac.uk.

Abstract

CRISPR/Cas9 is a rapidly developing genome editing technology that has been successfully applied in many organisms, including model and crop plants. Cas9, an RNA-guided DNA endonuclease, can be targeted to specific genomic sequences by engineering a separately encoded guide RNA with which it forms a complex. As only a short RNA sequence must be synthesized to confer recognition of a new target, CRISPR/Cas9 is a relatively cheap and easy to implement technology that has proven to be extremely versatile. Remarkably, in some plant species, homozygous knockout mutants can be produced in a single generation. Together with other sequence-specific nucleases, CRISPR/Cas9 is a game-changing technology that is poised to revolutionise basic research and plant breeding.

PMID:
25437637
DOI:
10.1016/j.copbio.2014.11.007
[Indexed for MEDLINE]

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