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Pathogens. 2014 Nov 3;3(4):875-907. doi: 10.3390/pathogens3040875.

Comparison of potato and asian citrus psyllid adult and nymph transcriptomes identified vector transcripts with potential involvement in circulative, propagative liberibacter transmission.

Author information

1
School of Plant Sciences, The University of Arizona, Tucson, AZ 85721, USA. twfisher@email.arizona.edu.
2
School of Plant Sciences, The University of Arizona, Tucson, AZ 85721, USA. molupapa@gmail.com.
3
Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA. rfhe@wsu.edu.
4
BIO5, The University of Arizona, Tucson, AZ 85721, USA. will@agcol.arizona.edu.
5
School of Plant Sciences, The University of Arizona, Tucson, AZ 85721, USA. jmc6@ag.arizona.edu.
6
BIO5, The University of Arizona, Tucson, AZ 85721, USA. mark@agcol.arizona.edu.
7
National Center for Genome Resources, 2935 Rodeo Park Drive East, Santa Fe, NM 87505, USA. rwkim@ucdavis.edu.
8
National Center for Genome Resources, 2935 Rodeo Park Drive East, Santa Fe, NM 87505, USA. gregory.may@pioneer.com.
9
National Center for Genome Resources, 2935 Rodeo Park Drive East, Santa Fe, NM 87505, USA. john.crow@pioneer.com.
10
BIO5, The University of Arizona, Tucson, AZ 85721, USA. cari@agcol.arizona.edu.
11
Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA. gangd@wsu.edu.
12
School of Plant Sciences, The University of Arizona, Tucson, AZ 85721, USA. jkbrown@email.arizona.edu.

Abstract

The potato psyllid (PoP) Bactericera cockerelli (Sulc) and Asian citrus psyllid (ACP) Diaphorina citri Kuwayama are the insect vectors of the fastidious plant pathogen, Candidatus Liberibacter solanacearum (CLso) and Ca. L. asiaticus (CLas), respectively. CLso causes Zebra chip disease of potato and vein-greening in solanaceous species, whereas, CLas causes citrus greening disease. The reliance on insecticides for vector management to reduce pathogen transmission has increased interest in alternative approaches, including RNA interference to abate expression of genes essential for psyllid-mediated Ca. Liberibacter transmission. To identify genes with significantly altered expression at different life stages and conditions of CLso/CLas infection, cDNA libraries were constructed for CLso-infected and -uninfected PoP adults and nymphal instars. Illumina sequencing produced 199,081,451 reads that were assembled into 82,224 unique transcripts. PoP and the analogous transcripts from ACP adult and nymphs reported elsewhere were annotated, organized into functional gene groups using the Gene Ontology classification system, and analyzed for differential in silico expression. Expression profiles revealed vector life stage differences and differential gene expression associated with Liberibacter infection of the psyllid host, including invasion, immune system modulation, nutrition, and development.

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