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Biomed Res Int. 2014;2014:410167. doi: 10.1155/2014/410167. Epub 2014 Nov 10.

Periodontal ligament mesenchymal stromal cells increase proliferation and glycosaminoglycans formation of temporomandibular joint derived fibrochondrocytes.

Author information

1
Department of Stomatology, The First Affiliated Hospital of Henan, University of Science and Technology, 24 Jinghua Road, Jianxi District, Luoyang, Henan 471003, China.
2
Department of Pathology, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang 471003, China.
3
Department of Oncology, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang 471003, China.

Abstract

OBJECTIVES:

Temporomandibular joint (TMJ) disorders are common disease in maxillofacial surgery. The aim of this study is to regenerate fibrocartilage with a mixture of TMJ fibrochondrocytes and periodontal ligament derived mesenchymal stem cells (PD-MSCs).

MATERIALS AND METHODS:

Fibrochondrocytes and PD-MSC were cocultured (ratio 1 : 1) for 3 weeks. Histology and glycosaminoglycans (GAGs) assay were performed to examine the deposition of GAG. Green florescent protein (GFP) was used to track PD-MSC. Conditioned medium of PD-MSCs was collected to study the soluble factors. Gene expression of fibrochondrocytes cultured in conditioned medium was tested by quantitative PCR (qPCR).

RESULTS:

Increased proliferation of TMJ-CH was observed in coculture pellets when compared to monoculture. Enhanced GAG production in cocultures was shown by histology and GAG quantification. Tracing of GFP revealed the fact that PD-MSC disappears after coculture with TMJ-CH for 3 weeks. In addition, conditioned medium of PD-MSC was also shown to increase the proliferation and GAG deposition of TMJ-CH. Meanwhile, results of qPCR demonstrated that conditioned medium enhanced the expression levels of matrix-related genes in TMJ-CH.

CONCLUSIONS:

Results from this study support the mechanism of MSC-chondrocyte interaction, in which MSCs act as secretor of soluble factors that stimulate proliferation and extracellular matrix deposition of chondrocytes.

PMID:
25436212
PMCID:
PMC4243606
DOI:
10.1155/2014/410167
[Indexed for MEDLINE]
Free PMC Article

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