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Nat Prod Res. 2015;29(22):2103-6. doi: 10.1080/14786419.2014.986660. Epub 2014 Dec 1.

Placental extracts induce the expression of antioxidant enzyme genes and suppress melanogenesis in B16 melanoma cells.

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a Department of Health Chemistry , Hoshi University , 2-4-41, Ebara, Shinagawa-Ku, Tokyo 142-8501 , Japan.
b Snowden Corporation , Chiyoda-Ku, Tokyo 101-0031 , Japan.


One of the activities of placental extracts (PEs) is skin-whitening effect, but the physiological and genetic mechanism for this effect has not yet been clarified. Here, we focus on PE as a regulator of antioxidant enzyme genes. Porcine PE was prepared, and its activity was investigated in B16 melanoma cells. PE treatment decreased the melanin content of UV-irradiated B16 cells in a dose-dependent manner. PE directly reduced the enzyme activity of tyrosinase in a cell-free assay. In addition, PE treatment increased the gene expression of cytosolic superoxide dismutase (SOD-1), extracellular SOD (SOD-3) and catalase but did not affect the expression of tyrosinase. Moreover, PE protected the B16 cells from H2O2-induced cell death. Taken together, our data suggest that PEs could play a role not only as a suppressor of melanin synthesis but also as a regulator of antioxidant genes and might protect the skin against oxidative stress.


antioxidant enzyme; melanin synthesis; placenta extracts; superoxide dismutase

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