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Sci Rep. 2014 Dec 1;4:7253. doi: 10.1038/srep07253.

Axial plane optical microscopy.

Author information

1
1] NSF Nano-scale Science and Engineering Center, 3112 Etcheverry Hall, University of California, Berkeley, California 94720, USA [2] Materials Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720, USA.
2
NSF Nano-scale Science and Engineering Center, 3112 Etcheverry Hall, University of California, Berkeley, California 94720, USA.

Abstract

We present axial plane optical microscopy (APOM) that can, in contrast to conventional microscopy, directly image a sample's cross-section parallel to the optical axis of an objective lens without scanning. APOM combined with conventional microscopy simultaneously provides two orthogonal images of a 3D sample. More importantly, APOM uses only a single lens near the sample to achieve selective-plane illumination microscopy, as we demonstrated by three-dimensional (3D) imaging of fluorescent pollens and brain slices. This technique allows fast, high-contrast, and convenient 3D imaging of structures that are hundreds of microns beneath the surfaces of large biological tissues.

PMID:
25434770
PMCID:
PMC4248283
DOI:
10.1038/srep07253
[Indexed for MEDLINE]
Free PMC Article

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