Format

Send to

Choose Destination
Toxicol In Vitro. 2015 Mar;29(2):308-13. doi: 10.1016/j.tiv.2014.11.008. Epub 2014 Nov 26.

The inflammation and estrogen metabolism impacts of polychlorinated biphenyls on endometrial cancer cells.

Author information

1
Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China; Ningbo Urban Environment Observation and Research Station-NUEORS, Chinese Academy of Sciences, PR China.
2
Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China; Ningbo Urban Environment Observation and Research Station-NUEORS, Chinese Academy of Sciences, PR China. Electronic address: qshuang@iue.ac.cn.
3
First Affiliated Hospital of Xiamen University, Xiamen 361003, PR China.
4
Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China; Ningbo Urban Environment Observation and Research Station-NUEORS, Chinese Academy of Sciences, PR China. Electronic address: sjdong@iue.ac.cn.

Abstract

Polychlorinated biphenyls (PCBs) are persistent and bio-accumulative chemicals that provoke a wide range of toxic effects. Their adverse impacts on the reproductive system are of great concern, however, the effects of PCBs on endometrium are still unclear. In the study, the endometrial adenocarcinoma Ishikawa cells were exposed to both dioxin-like CB126 and non-dioxin-like CB153 at the nominal concentrations of 0.3, 3, and 30μM. The inflammatory and endocrine effects were detected after treatment by PCBs. Results showed that CB126 stimulated the proliferation of Ishikawa cells at lower concentrations of 0.3 and 3μM. By contrast, CB153 did not affect the viability of the cells. Both congeners exerted the stimulatory effects on the enzymatic activity of SOD1. CB126 decreased the abundance of Interleukin-8 both at the mRNA and protein levels. Blocking of estrogen receptor or aryl hydrocarbon receptor by the antagonist abolished the effects of CB126 on the expressions of inflammatory factors. The levels of testosterone and 17beta-estradiol were not changed after exposure to lower doses of PCBs. In accordance, PCBs did not affect the mRNA expressions of estrogen metabolism-related genes. In all, our study revealed that PCBs affected the expression of inflammatory factors through ER and AHR receptors, however, no toxic effects were observed on estrogen metabolism.

KEYWORDS:

CB126; Endocrine; Estrogen metabolism; Inflammation; Ishikawa cells; Polychlorinated biphenyls

PMID:
25433332
DOI:
10.1016/j.tiv.2014.11.008
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center