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Pharm Biol. 2015 May;53(5):662-71. doi: 10.3109/13880209.2014.936470. Epub 2014 Nov 28.

Anti-inflammatory and antioxidant properties of unripe papaya extract in an excision wound model.

Author information

1
Department of Physiology, Faculty of Basic Medical Sciences, College of Health Science, University of Ilorin , Ilorin , Nigeria and.

Abstract

CONTEXT:

Carica papaya L. (Caricaceae) fruit was shown to exhibit wound healing properties.

OBJECTIVES:

We investigated anti-inflammatory and antioxidant potential of papaya fruit phosphate-buffered saline extract (PE) during wound healing and enhancement of the potentials due to trace ions addition.

MATERIALS AND METHODS:

Rat excision wounds were topically treated twice/day with 20 µL of PE (5 mg extract/mL), 0.5 µg Se(2+) added PE (PES), or 100 µM Zn(2+) added PE (PEZ). Control groups were treated with deionized water (negative) and deproteinized calf blood extract ointment (Solcoseryl®, positive). Lipid peroxidation (LPX), antioxidant, proinflammatory, and arginine metabolic enzymes were estimated in the wound excised on days 4 and 10 post wounding.

RESULTS:

PE (5 mg/mL; 9.80 ± 0.33 d) and PES (PE + 0.5 µg Se(2+); 8.90 ± 0.23 d) significantly (p < 0.05) reduced the average time for complete wound closure compared with the negative (13.00 ± 0.37 d) and positive (9.80 ± 0.33 d) controls, respectively. Biochemical evaluations of LPX product (malondialdehyde), antioxidant (catalase, superoxide dismutase (SOD), and glutathione peroxidase (GPx)), and pro-inflammatory (cyclooxygenase-2 and myeloperoxidase (MPO)) enzyme activities and metabolites (nitrite and urea), on days 4 and 10 post wounding, confirmed the anti-inflammatory and antioxidant properties of PE and PES in this study.

DISCUSSION AND CONCLUSION:

Treatment of excision wounds with papaya extract, especially with the addition of selenium for 10 d, reduced inflammation associated oxidative damage apparently via cyclooxygenase specific inhibition, arginine metabolism, and up-regulation of antioxidant enzymes.

KEYWORDS:

Antioxidant enzyme; arginine metabolism; lipid peroxidation; proinflammatory enzymes; selenium; wound healing

PMID:
25431122
DOI:
10.3109/13880209.2014.936470
[Indexed for MEDLINE]

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