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Proteomics. 2015 Mar;15(5-6):1026-38. doi: 10.1002/pmic.201400310. Epub 2015 Jan 21.

Current peptidomics: applications, purification, identification, quantification, and functional analysis.

Author information

1
Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis, CA, USA; Foods for Health Institute, University of California, Davis, One Shields Avenue, Davis, CA, USA.

Abstract

Peptidomics is an emerging field branching from proteomics that targets endogenously produced protein fragments. Endogenous peptides are often functional within the body-and can be both beneficial and detrimental. This review covers the use of peptidomics in understanding digestion, and identifying functional peptides and biomarkers. Various techniques for peptide and glycopeptide extraction, both at analytical and preparative scales, and available options for peptide detection with MS are discussed. Current algorithms for peptide sequence determination, and both analytical and computational techniques for quantification are compared. Techniques for statistical analysis, sequence mapping, enzyme prediction, and peptide function, and structure prediction are explored.

KEYWORDS:

Functional prediction; MS; Peptidomics; Quantitation; Structure prediction; Systems biology

PMID:
25429922
PMCID:
PMC4371869
DOI:
10.1002/pmic.201400310
[Indexed for MEDLINE]
Free PMC Article

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