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Elife. 2014 Nov 27;3. doi: 10.7554/eLife.03197.

A bacteriophage tubulin harnesses dynamic instability to center DNA in infected cells.

Author information

1
Division of Biological Sciences, University of California, San Diego, La Jolla, United States.
2
Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, United States.

Abstract

Dynamic instability, polarity, and spatiotemporal organization are hallmarks of the microtubule cytoskeleton that allow formation of complex structures such as the eukaryotic spindle. No similar structure has been identified in prokaryotes. The bacteriophage-encoded tubulin PhuZ is required to position DNA at mid-cell, without which infectivity is compromised. Here, we show that PhuZ filaments, like microtubules, stochastically switch from growing in a distinctly polar manner to catastrophic depolymerization (dynamic instability) both in vitro and in vivo. One end of each PhuZ filament is stably anchored near the cell pole to form a spindle-like array that orients the growing ends toward the phage nucleoid so as to position it near mid-cell. Our results demonstrate how a bacteriophage can harness the properties of a tubulin-like cytoskeleton for efficient propagation. This represents the first identification of a prokaryotic tubulin with the dynamic instability of microtubules and the ability to form a simplified bipolar spindle.

KEYWORDS:

bacteriophage; cell biology; cytoskeleton; dynamic instability; pseudomonas; spindle; tubulin

PMID:
25429514
PMCID:
PMC4244570
DOI:
10.7554/eLife.03197
[Indexed for MEDLINE]
Free PMC Article

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