Format

Send to

Choose Destination
DNA Res. 2015 Feb;22(1):101-7. doi: 10.1093/dnares/dsu043. Epub 2014 Nov 26.

A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster.

Author information

1
Department of Animal and Grassland Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan iguchi@med.miyazaki-u.ac.jp.
2
Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
3
Division of Parasitology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Miyazaki 889-1692, Japan.
4
Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Miyazaki 889-1692, Japan Division of Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki, Miyazaki 889-1692, Japan.
5
Division of Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki, Miyazaki 889-1692, Japan.
6
Pathogen Genomics, The Wellcome Trust Sanger Institute, Cambridge CB10 1SA, UK Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK.

Abstract

The O antigen constitutes the outermost part of the lipopolysaccharide layer in Gram-negative bacteria. The chemical composition and structure of the O antigen show high levels of variation even within a single species revealing itself as serological diversity. Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups. By comparing these sequences, we identified 161 well-defined O-AGCs. Based on the wzx/wzy or wzm/wzt gene sequences, in addition to 145 singletons, 37 serogroups were placed into 16 groups. Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs. Our data provide a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized. These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.

KEYWORDS:

E. coli; O serogroup; O-antigen biosynthesis gene cluster; genomic diversity; horizontal gene transfer

PMID:
25428893
PMCID:
PMC4379981
DOI:
10.1093/dnares/dsu043
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center