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Arch Virol. 2015 Feb;160(2):617-9. doi: 10.1007/s00705-014-2288-8. Epub 2014 Nov 27.

Molecular identification of a new begomovirus associated with leaf crumple disease of Jatropha curcas L. in India.

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Plant Molecular Virology Laboratory, CPMB Division, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226001, UP, India.


Association of a new begomovirus with leaf crumple disease of Jatropha curcas was identified based on sequence analysis of cloned ~2.7-kb viral DNA genomes from three representative samples amplified by RCA using phi-29 DNA polymerase. Sequence analysis of these isolates SKJ1 (KM066975), SKJ2 (KM189818) and SKJ3 (KM189819)--showed the presence of a monopartite begomovirus-like genome of 2,735 to 2,738 nucleotides containing seven ORFs: V3, V2 and V1 in virion sense and C3, C2, C1 and C4 in the complementary sense. Pairwise alignment showed 99 % nucleotide sequence similarity to each other and less than 81 % identity to other begomoviruses reported worldwide. Based on begomovirus species demarcation criteria for a new species (sequence identity <89 %), the begomovirus isolates were identified as the members of a new Begomovirus species and provisionally designated as Jatropha leaf crumple India virus (JLCrIV).

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