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J Virol Methods. 1989 Feb;23(2):157-67.

A rapid microneutralization assay for the measurement of neutralizing antibody reactive with human cytomegalovirus.

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Department of Infectious Diseases, 2nd University of Rome, Italy.


We have developed a murine monoclonal antibody reactive with a major immediate early 72,000 dalton protein of human cytomegalovirus and utilized this reagent in a rapid virus titration and microneutralization assay. Because of the early expression of this virus encoded protein, both assays could be accomplished within 16 h following virus inoculation. In addition, both assays resulted in considerable savings of reagents because the assays were carried out in 96-well microtiter plates. These assays should prove useful in the preparation and study of neutralizing antibodies directed against human cytomegalovirus.

[Indexed for MEDLINE]

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