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Nano Lett. 2015 Jan 14;15(1):103-6. doi: 10.1021/nl503058k. Epub 2014 Dec 2.

Dual channel RESOLFT nanoscopy by using fluorescent state kinetics.

Author information

1
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry , Göttingen, Germany.

Abstract

We show that RESOLFT fluorescence nanoscopy, a low light level scanning superresolution technique employing reversibly switchable fluorescent proteins (rsFPs), is capable of dual-channel live-cell imaging that is virtually free of chromatic errors and temporal offsets. This is accomplished using rsEGFP and Dronpa, two rsFPs having similar spectra but different kinetics of switching and fluorescence emission. Our approach is demonstrated by imaging protein distributions and dynamics in living neurons and neuronal tissues.

KEYWORDS:

Photoswitching; lifetime; low intensities; nanoscopy

PMID:
25423166
DOI:
10.1021/nl503058k
[Indexed for MEDLINE]

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