Format

Send to

Choose Destination
Clin Chem. 1989 May;35(5):835-7.

A new commercial method for the enzymatic determination of creatinine in serum and urine evaluated: comparison with a kinetic Jaffé method and isotope dilution-mass spectrometry.

Author information

1
Department of Clinical Chemistry, Huddinge University Hospital, Sweden.

Abstract

We evaluated a new, simple, enzymatic kinetic method from Wako Chemicals GmbH in comparison with a kinetic Jaffé method by using isotope dilution-mass spectrometry (ID-MS) as a reference method. An ID-MS-calibrated serum standard was used. Both the enzymatic and the Jaffé method correlated well with ID-MS, except for sera with high concentrations of bilirubin. Ethyl acetoacetate, acetone, and glucose in serum interfered somewhat with the Jaffé method but not with the enzymatic method. We conclude that the present enzymatic method has merit as compared with a Jaffé method for routine work, but is more expensive.

PMID:
2541950
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center