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Nat Commun. 2014 Nov 24;5:5486. doi: 10.1038/ncomms6486.

TALEN and CRISPR/Cas9-mediated genome editing in the early-branching metazoan Nematostella vectensis.

Author information

1
Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, Missouri 64110, USA.
2
1] Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, Missouri 64110, USA [2] Department of Anatomy and Cell Biology, University of Kansas School of Medicine, Kansas City, Kansas 66160, USA.

Abstract

Non-bilaterian phyla represent key lineages for exploring the evolutionary history of early animals. However, despite an increasing number of sequenced genomes from early-branching metazoans, efficient and reproducible methodologies for analysis of gene function remain a major challenge. Here we report the utilization of the TALEN and CRISPR/Cas9 systems to induce targeted mutations and homologous recombination-mediated transgenesis in the sea anemone Nematostella vectensis. We also present a new method to isolate genetically modified animals using engineered selection cassettes introduced by homologous recombination. Taken together, these methods will permit sophisticated gain- and loss-of-function analyses in Nematostella and perhaps other early metazoan species that allow for zygotic injection.

PMID:
25417547
DOI:
10.1038/ncomms6486
[Indexed for MEDLINE]

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