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Cell. 2014 Nov 6;159(4):844-56. doi: 10.1016/j.cell.2014.10.032.

A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis.

Author information

1
Department of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Warren Alpert 524, Boston, MA 02115, USA.
2
Department of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Warren Alpert 524, Boston, MA 02115, USA. Electronic address: marc@hms.harvard.edu.
3
Department of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Warren Alpert 524, Boston, MA 02115, USA. Electronic address: gavin_macbeath@harvard.edu.

Abstract

Wnt signaling plays a critical role in embryonic development, and genetic aberrations in this network have been broadly implicated in colorectal cancer. We find that the Wnt receptor Frizzled2 (Fzd2) and its ligands Wnt5a/b are elevated in metastatic liver, lung, colon, and breast cancer cell lines and in high-grade tumors and that their expression correlates with markers of epithelial-mesenchymal transition (EMT). Pharmacologic and genetic perturbations reveal that Fzd2 drives EMT and cell migration through a previously unrecognized, noncanonical pathway that includes Fyn and Stat3. A gene signature regulated by this pathway predicts metastasis and overall survival in patients. We have developed an antibody to Fzd2 that reduces cell migration and invasion and inhibits tumor growth and metastasis in xenografts. We propose that targeting this pathway could provide benefit for patients with tumors expressing high levels of Fzd2 and Wnt5a/b.

PMID:
25417160
PMCID:
PMC4243058
DOI:
10.1016/j.cell.2014.10.032
[Indexed for MEDLINE]
Free PMC Article

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