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Cell. 2014 Oct 23;159(3):543-57. doi: 10.1016/j.cell.2014.09.033.

Enhancer interaction networks as a means for singular olfactory receptor expression.

Author information

1
Neuroscience Graduate Program, University of California, San Francisco, San Francisco, CA 94158, USA.
2
Neuroscience Graduate Program, Stanford University School of Medicine, Stanford, CA 94305, USA.
3
Department of Anatomy, University of California, San Francisco, San Francisco, CA 94158, USA.
4
Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA 94158, USA; Institute for Human Genetics, University of California, San Francisco, San Francisco, CA 94158, USA.
5
Neuroscience Graduate Program, University of California, San Francisco, San Francisco, CA 94158, USA; Department of Anatomy, University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address: sl682@columbia.edu.

Abstract

The transcriptional activation of one out of ?2800 olfactory receptor (OR) alleles is a poorly understood process. Here, we identify a plethora of putative OR enhancers and study their in vivo activity in olfactory neurons. Distinguished by an unusual epigenetic signature, candidate OR enhancers are characterized by extensive interchromosomal interactions associated with OR transcription and share a similar pattern of transcription factor footprints. In particular, we establish the role of the transcription factor Bptf as a facilitator of both enhancer interactions and OR transcription. Our observations agree with the model whereby OR transcription occurs in the context of multiple interacting enhancers. Disruption of these interchromosomal interactions results in weak and multigenic OR expression, suggesting that the rare coincidence of numerous enhancers over a stochastically chosen OR may account for the singularity and robustness in OR transcription.

PMID:
25417106
PMCID:
PMC4243057
DOI:
10.1016/j.cell.2014.09.033
[Indexed for MEDLINE]
Free PMC Article

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