(A) Evaluation of the quality of Co-Frac, PrePPI-HC and pairs from small-scale experiments in the literature with no binary evidence (Lit-NB-13). Fraction of pairs recovered by Y2H as compared to pairs from Lit-BM-13 and pairs of randomly selected proteins (RRS) (left). Enrichment in functional interactions and co-complex memberships (right). Legend as in . For n values, see .
(B) Adjacency matrices for HI-II-14, Lit-BM-13, Co-Frac and PrePPI-HC maps, with proteins per bins of ~350 and ordered by number of publications, mRNA abundance in HEK cells, fraction of protein sequence covered by Pfam domains, or fraction of protein sequence in transmembrane helices. Figure legend as in .
(C) Highest interaction density imbalances (observed minus expected) are shown in the four maps, the union of all four maps, and our previous binary map (HI-I-05) for 21 protein properties.
(D) Precision at 1% RRS recovery in the MAPPIT assay (top, error bars indicate standard error of the proportion) and functional enrichment (bottom, union of Gene Ontology and mouse phenotypes based annotations, error bars indicate 95% confidence intervals) of HI-II-14 pairs found in dense and sparse zones mirrored from Lit-BM-13, Co-Frac and PrePPI-HC. P > 0.05 for all pairwise comparisons of dense and sparse zones, two-sided Fisher’s exact tests. For n values, see .
See also and .