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Methods Mol Biol. 2015;1224:25-35. doi: 10.1007/978-1-4939-1658-0_3.

Jatropha (Jatropha curcas L.).

Author information

1
Center for Energy, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.

Abstract

The seed oil of Jatropha (Jatropha curcas L.) as a source of biodiesel fuel is gaining worldwide importance. Commercial-scale exploration of Jatropha has not succeeded due to low and unstable seed yield in semiarid lands unsuitable for the food production and infestation to diseases. Genetic engineering is promising to improve various agronomic traits in Jatropha and to understand the molecular functions of key Jatropha genes for molecular breeding. We describe a protocol routinely followed in our laboratory for stable and efficient Agrobacterium tumefaciens-mediated transformation of Jatropha using cotyledonary leaf as explants. The 4-day-old explants are infected with Agrobacterium tumefaciens strain EHA105 harboring pBI121 plant binary vector, which contains nptII as plant selectable marker and gus as reporter. The putative transformed plants are selected on kanamycin, and stable integration of transgene(s) is confirmed by histochemical GUS assay, polymerase chain reaction, and Southern hybridization.

PMID:
25416246
DOI:
10.1007/978-1-4939-1658-0_3
[Indexed for MEDLINE]

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