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J Med Chem. 2014 Dec 26;57(24):10355-65. doi: 10.1021/jm501058g. Epub 2014 Dec 5.

Novel insights into structure and function of factor XIIIa-inhibitor tridegin.

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1
Pharmaceutical Chemistry I, Institute of Pharmacy, University of Bonn , Brühler Str. 7, 53119 Bonn, Germany.

Abstract

The inhibition of the final step in blood coagulation, the factor XIIIa (FXIIIa) catalyzed cross-linking of fibrin monomers, is currently still a challenge in medicinal chemistry. We report synthesis, recombinant expression, disulfide connectivity, and biological activity of tridegin, the sole existing peptide representative displaying inhibitory activity on FXIIIa. Inhibition of the enzyme by this 66-mer cysteine-rich peptide is mediated by its C-terminal sequence, while the N-terminal part comprises structural information and contributes to inhibitor binding. Either of the production strategies examined leads to the formation of different disulfide-bridged isomers indicating the requirement of the correct fold for inhibitory activity. Molecular modeling and docking studies confirm disulfide bond isomer preference with respect to binding to FXIIIa, in turn, the knowledge of the enzyme-inhibitor interactions might bring about comprehensive ideas for the design of a suitable lead structure for addressing FXIIIa.

PMID:
25415134
DOI:
10.1021/jm501058g
[Indexed for MEDLINE]

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