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Nat Commun. 2014 Nov 21;5:5511. doi: 10.1038/ncomms6511.

Epithelial rotation promotes the global alignment of contractile actin bundles during Drosophila egg chamber elongation.

Author information

1
1] Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA [2] Committee on Development, Regeneration and Stem Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA.
2
1] Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA [2] Institute for Biophysical Dynamics, James Franck Institute and Department of Physics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
3
Institute for Biophysical Dynamics, James Franck Institute and Department of Physics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
4
1] Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA [2] Department of Biological Sciences, Butler University, 4600 Sunset Boulevard, Indianapolis, Indiana 46208, USA.
5
Life Sciences Centre, Department of Cellular and Physiological Sciences, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia, Canada V6T 1Z3.

Abstract

Tissues use numerous mechanisms to change shape during development. The Drosophila egg chamber is an organ-like structure that elongates to form an elliptical egg. During elongation the follicular epithelial cells undergo a collective migration that causes the egg chamber to rotate within its surrounding basement membrane. Rotation coincides with the formation of a 'molecular corset', in which actin bundles in the epithelium and fibrils in the basement membrane are all aligned perpendicular to the elongation axis. Here we show that rotation plays a critical role in building the actin-based component of the corset. Rotation begins shortly after egg chamber formation and requires lamellipodial protrusions at each follicle cell's leading edge. During early stages, rotation is necessary for tissue-level actin bundle alignment, but it becomes dispensable after the basement membrane is polarized. This work highlights how collective cell migration can be used to build a polarized tissue organization for organ morphogenesis.

PMID:
25413675
PMCID:
PMC4241503
DOI:
10.1038/ncomms6511
[Indexed for MEDLINE]
Free PMC Article

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