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Mol Biol Cell. 2015 Jan 15;26(2):373-86. doi: 10.1091/mbc.E14-11-1503. Epub 2014 Nov 19.

Mechanisms of expression and translocation of major fission yeast glucose transporters regulated by CaMKK/phosphatases, nuclear shuttling, and TOR.

Author information

1
Institute of Life Science, Kurume University, Hyakunen-Kohen 1-1, Kurume, Fukuoka 839-0864, Japan saitou_shigeaki@kurume-u.ac.jp myanagid@gmail.com.
2
Okinawa Institute Science and Technology Graduate University, Tancha 1919-1, Onna, Okinawa 904-0495, Japan.
3
Institute of Life Science, Kurume University, Hyakunen-Kohen 1-1, Kurume, Fukuoka 839-0864, Japan.
4
Okinawa Institute Science and Technology Graduate University, Tancha 1919-1, Onna, Okinawa 904-0495, Japan saitou_shigeaki@kurume-u.ac.jp myanagid@gmail.com.

Abstract

Hexose transporters are required for cellular glucose uptake; thus they play a pivotal role in glucose homeostasis in multicellular organisms. Using fission yeast, we explored hexose transporter regulation in response to extracellular glucose concentrations. The high-affinity transporter Ght5 is regulated with regard to transcription and localization, much like the human GLUT transporters, which are implicated in diabetes. When restricted to a glucose concentration equivalent to that of human blood, the fission yeast transcriptional regulator Scr1, which represses Ght5 transcription in the presence of high glucose, is displaced from the nucleus. Its displacement is dependent on Ca(2+)/calmodulin-dependent kinase kinase, Ssp1, and Sds23 inhibition of PP2A/PP6-like protein phosphatases. Newly synthesized Ght5 locates preferentially at the cell tips with the aid of the target of rapamycin (TOR) complex 2 signaling. These results clarify the evolutionarily conserved molecular mechanisms underlying glucose homeostasis, which are essential for preventing hyperglycemia in humans.

PMID:
25411338
PMCID:
PMC4294683
DOI:
10.1091/mbc.E14-11-1503
[Indexed for MEDLINE]
Free PMC Article

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